12. Thomas Holford of Holford, Esq. son and heir of Sir John, married Margaret daughter of Sir Thomas Butler of Bewsy, in Lancashire, near Warrington, by whom he had issue Christopher, son and heir. After the death of Margaret, Thomas married Jane the widow of Hugh Dutton, son and heir of Sir Piers Dutton of Dutton and Hatton both, and daughter of Sir William Booth of Dunham Massy; by whom he had issue George Holford of Newborough in Dutton, gentleman; Thomas and John; also Ellen, married to John Carrington of Carrington in Cheshire, Esq.; Dorothy, married to John Bruyn of Stapleford in Cheshire, Esq. and Elizabeth, married to Charles Manwaring of Croxton in Cheshire, Esq. 1560. Thomas died 24 September 1569.