13. Christopher Holford of Holford, Esq. son and heir of Thomas, had also two wives. The first was Anne, daughter of Hugh Dutton and Jane aforesaid, (thus he married his stepsister) by whom he had issue Thomas Holford. John and Ann (presumably two additional children) who died young. The second wife of Christopher was Elizabeth the widow of Peter Shakerley of Houlme juxta Nether-Peover, the daughter and co-heir of Sir Randle Manwaring of Over Peover. They married 13 July 1561 and had issue Mary Holford, baptised at Nether Peover, 20 Jan 1562, who became sole heir to her father. Christopher died 27 January 1581.